Citation information for individual datasets is often provided in the metadata. However, not all datasets have this information embedded in the discovery metadata. On a general basis a citation of a dataset include the same components as any other citation:
author,
title,
year of publication,
publisher (for data this is often the archive where it is housed),
edition or version,
access information (a URL or persistent identifier, e.g. DOI if provided)
The information required to properly cite a dataset is normally provided in the discovery metadata the datasets.
If you use data retrieved through this portal, please acknowledge the SAON Data Portal.
Brief user guide
The Data Access Portal has information in 3 columns. An outline of the content in these columns is provided above. When first entering the search interface, all potential datasets are listed. Datasets are indicated in the map and results tabulation elements which are located in the middle column. The order of results can be modified using the "Sort by" option in the left column. On top of this column is normally relevant guidance information to user presented as collapsible elements.
If the user want to refine the search, this can be done by constraining the bounding box search. This is done in the map - the listing of datasets is automatically updated. Date constraints can be added in the left column. For these to take effect, the user has to push the button marked search. In the left column it is also possible to specific text elements to search for in the datasets. Again pushing the button marked "Search" is necessary for these to take action. Complex search patterns can be constructed using logical operators through the drop down menu above the text field. Text strings that are not quoted are treated as separate words and will match any of the words (i.e. assuming the OR operator). Phrases may be prefixed with '-' to indicate no occurence of the phrase in the results.
Other elements indicated in the left and right columns are facet searches, i.e. these are keywords that are found in the datasets and all datasets that contain these specific keywords in the appropriate metadata elements are listed together. Further refinement can be done using full text, date or bounding box constraints. Individuals, organisations and data centres involved in generating or curating the datasets are listed in the facets in the right column.
This dataset compiles Fatty Acid composition (relative proportions) of 4-day old Calanus hyperboreus nauplii from mothers collected in the Northern Barents Sea. Gravid Calanus hyperboreus females were collected from the Northern Barents Sea during the Q1 Nansen Legacy Seasonal Cruise in March 2021. After hatching, 4-day old Calanus hyperboreus nauplii were used for a short-term incubation assay (24h), exposing the nauplii to acidification (pH 8.01 and 7.50) and warming (0°C and 3°C), both individually and in combination. Nauplii larvae were added to each replicate at a density of ~0.6 larvae mL-1. The bottles were kept in the dark in incubators at the target temperature for 24 hours. After the incubation, all larvae from each treatment were extracted and checked for survival, and the remaining individuals were stored in Eppendorf tubes, freeze-dried, and kept at -80°C for lipid content analysis.
Quality
The lipid content and fatty acid compositions of the copepod nauplii obtained from the incubation assays were analysed at the Alfred Wegener Institute in Bremerhaven, Germany. To acquire sufficient sample material for the analysis, between 100 to 148 individual larvae were pooled per sample. Triplicate samples (except for Treatment 3, which had duplicates) were analysed for each treatment. Before lipid extraction, the samples were freeze-dried for 24 h at -80 °C and then mechanically homogenized using a Potter-Elvehjem homogenizer. Total lipids were extracted by using a modified protocol from Folch et al., (1957), with dichloromethane/methanol (2:1, v/v), followed by cleaning with 0.88% potassium chloride solution. The extracted lipids were then transformed into fatty acid methyl esters (FAMEs) and free fatty alcohols derived from wax esters by transesterification in methanol containing 3% concentrated sulfuric acid, at 80 °C for 4 h. The FAMEs and alcohols were separated via an Agilent 6890N Network gas chromatograph (Agilent Technologies, USA) with a DB-FFAP capillary column (30 m, 0.25 mm I.D., 0.25 µm film thickness), equipped with a flame-ionization detector using a temperature program (160 to 240 °C). The samples were injected at 160 °C with helium as the carrier gas. The FAMEs were identified using standard mixtures, and the total lipid content was quantified as the sum of FAs and fatty alcohols using an internal standard (23:0) that was added prior to lipid extraction. The fatty acids are expressed in the nomenclature A:Bn-X, where A represents the number of carbon atoms, B the amount of double bonds, and X is the position of the first double bond starting from the methyl end of the carbon chain. The proportions of individual FAs are expressed as mass percentages of the total FA content.